Fungal β-glucosidase expression in Saccharomyces cerevisiae.
Identifieur interne : 000437 ( Main/Exploration ); précédent : 000436; suivant : 000438Fungal β-glucosidase expression in Saccharomyces cerevisiae.
Auteurs : A P Njokweni [Afrique du Sud] ; S H Rose ; W H Van ZylSource :
- Journal of industrial microbiology & biotechnology [ 1476-5535 ] ; 2012.
Descripteurs français
- KwdFr :
- Anaérobiose (MeSH), Cellobiose (métabolisme), Cellulose (métabolisme), Chromatographie en phase liquide à haute performance (MeSH), Fermentation (MeSH), Kluyveromyces (enzymologie), Kluyveromyces (génétique), Phanerochaete (enzymologie), Phanerochaete (génétique), Protéines de transport membranaire (génétique), Protéines de transport membranaire (métabolisme), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Thermoascus (enzymologie), Thermoascus (génétique), bêta-Glucosidase (biosynthèse), bêta-Glucosidase (génétique), bêta-Glucosidase (métabolisme), Éthanol (métabolisme), Éthanol (ressources et distribution).
- MESH :
- biosynthèse : Protéines recombinantes, bêta-Glucosidase.
- croissance et développement : Saccharomyces cerevisiae.
- enzymologie : Kluyveromyces, Phanerochaete, Thermoascus.
- génétique : Kluyveromyces, Phanerochaete, Protéines de transport membranaire, Protéines recombinantes, Saccharomyces cerevisiae, Thermoascus, bêta-Glucosidase.
- métabolisme : Cellobiose, Cellulose, Protéines de transport membranaire, Protéines recombinantes, Saccharomyces cerevisiae, bêta-Glucosidase, Éthanol.
- ressources et distribution : Éthanol.
- Anaérobiose, Chromatographie en phase liquide à haute performance, Fermentation.
English descriptors
- KwdEn :
- Anaerobiosis (MeSH), Cellobiose (metabolism), Cellulose (metabolism), Chromatography, High Pressure Liquid (MeSH), Ethanol (metabolism), Ethanol (supply & distribution), Fermentation (MeSH), Kluyveromyces (enzymology), Kluyveromyces (genetics), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Thermoascus (enzymology), Thermoascus (genetics), beta-Glucosidase (biosynthesis), beta-Glucosidase (genetics), beta-Glucosidase (metabolism).
- MESH :
- chemical , biosynthesis : Recombinant Proteins, beta-Glucosidase.
- chemical , genetics : Membrane Transport Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , metabolism : Cellobiose, Cellulose, Ethanol, Membrane Transport Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , supply & distribution : Ethanol.
- enzymology : Kluyveromyces, Phanerochaete, Thermoascus.
- genetics : Kluyveromyces, Phanerochaete, Saccharomyces cerevisiae, Thermoascus.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Anaerobiosis, Chromatography, High Pressure Liquid, Fermentation.
Abstract
Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.
DOI: 10.1007/s10295-012-1150-9
PubMed: 22707073
Affiliations:
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qualifier="métabolisme" xml:lang="fr"><term>Cellobiose</term><term>Cellulose</term><term>Protéines de transport membranaire</term><term>Protéines recombinantes</term><term>Saccharomyces cerevisiae</term><term>bêta-Glucosidase</term><term>Éthanol</term></keywords><keywords scheme="MESH" qualifier="ressources et distribution" xml:lang="fr"><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Anaerobiosis</term><term>Chromatography, High Pressure Liquid</term><term>Fermentation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anaérobiose</term><term>Chromatographie en phase liquide à haute performance</term><term>Fermentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">22707073</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1476-5535</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>10</Issue><PubDate><Year>2012</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of industrial microbiology & biotechnology</Title><ISOAbbreviation>J Ind Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Fungal β-glucosidase expression in Saccharomyces cerevisiae.</ArticleTitle><Pagination><MedlinePgn>1445-52</MedlinePgn></Pagination><Abstract><AbstractText>Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Njokweni</LastName><ForeName>A P</ForeName><Initials>AP</Initials><AffiliationInfo><Affiliation>Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rose</LastName><ForeName>S H</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>van Zyl</LastName><ForeName>W H</ForeName><Initials>WH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Ind Microbiol Biotechnol</MedlineTA><NlmUniqueID>9705544</NlmUniqueID><ISSNLinking>1367-5435</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026901">Membrane Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>16462-44-5</RegistryNumber><NameOfSubstance UI="D002475">Cellobiose</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9068-45-5</RegistryNumber><NameOfSubstance UI="C021499">lactose permease</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.21</RegistryNumber><NameOfSubstance UI="D001617">beta-Glucosidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000693" MajorTopicYN="N">Anaerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002475" MajorTopicYN="N">Cellobiose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000600" MajorTopicYN="N">supply & distribution</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007716" MajorTopicYN="N">Kluyveromyces</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D026901" MajorTopicYN="N">Membrane Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055327" MajorTopicYN="N">Thermoascus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001617" MajorTopicYN="N">beta-Glucosidase</DescriptorName><QualifierName UI="Q000096" 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IdType="pubmed">12209002</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><noCountry><name sortKey="Rose, S H" sort="Rose, S H" uniqKey="Rose S" first="S H" last="Rose">S H Rose</name><name sortKey="Van Zyl, W H" sort="Van Zyl, W H" uniqKey="Van Zyl W" first="W H" last="Van Zyl">W H Van Zyl</name></noCountry><country name="Afrique du Sud"><noRegion><name sortKey="Njokweni, A P" sort="Njokweni, A P" uniqKey="Njokweni A" first="A P" last="Njokweni">A P Njokweni</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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